Keberadaan Escherichia coli dan Salmonella pada Daging Ayam Broiler yang Diberi Citrus limon dengan Variasi Suhu

Nadya Treesna Wulansari, A A Istri Mas Padwismari, Ni Made Raningsih

Abstract


Penelitian dilakukan untuk mengetahui keberadaan Escherichia coli dan Salmonella pada daging ayam broiler yang diberi air jeruk lemon (Citrus limon) dengan variasi suhu. Rancangan eksperimental penelitian digunakan dengan Rancangan Acak Lengkap. Daging ayam yang bersih direndam dengan air jeruk lemon selama 30 menit lalu disimpan selama 3x24 jam pada suhu 40C dan 250C. Selanjutnya dilakukan analisis total mikroba dan bakteri E.coli dan Salmonella. Hasil penelitian menunjukkan jumlah angka kuman tertinggi adalah pada perlakuan sampel daging ayam yang disimpan pada suhu 250C tanpa pemberian air jeruk lemon yaitu 6,24 x 105 CFU/ml. Sedangkan perlakuan terendah pada sampel daging ayam yang disimpan pada suhu 40C dengan pemberian jeruk lemon yaitu 6,2 x 102 CFU/ml. Suhu rendah dan kandungan fitokimia pada jeruk lemon berpotensi mampu menghambat pertumbuhan bakteri.

 

 


Keywords


daging ayam, E.coli, Salmonella, variasi suhu

References


Ariani, I.D (2017). Gambaran Air Perasan Jeruk Lemon (Citrus Limon (L) Brum.f.) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus. Karya Tulis Ilmiah. STIKES Insan Cendekia Medika. Jombang

Arlita, Y., Rares, F.E.S., Soeliongan, S. (2014). Identifikasi Bakteri Escherichia Coli Dan Salmonella Sp. Pada Makanan Jajanan Bakso Tusuk Di Kota Manado. eBIOMEDIK 2 (1).

Badan Standardisasi Nasional (2009). Batas Maksimus Cemaran Mikroba dalam Pangan. Badan Standardisasi Nasional.

Bahri, S., Rokhim, S., Prasiska, Y.S. (2019). Contamination of Escherichia coli Bacteria in Meat Samples. Journal of Health Science and Prevention 3 (1), 62-67.

Berti, P.L. (2015). Antibacterial Lemon (Citrus limon (L.) Burm.f)) on Porphyromonas gingivalis Domonant Periodontitis. Pbication Script. Faculty of Dentistry. Muhamadiyah University. Surakarta

Carraminana, J.J., Rota,C., Agustin, I., Herrera. A. (2004). High prevalence of multiple resistance to antibiotics in Salmonella serovars isolated from a poultry slaughterhouse in Spain. Vet Microbiol 104(1-2), 133-139.

Dewi, K.E.K., Habibah, N., Mastra, N. (2020). Uji Daya Hambat Berbagai Konsentrasi Perasan Jeruk Lemon Terhadap Bakteri Propionibacterium Acnes. Jurnal Sains dan Teknologi 9 (1),86-93.

Ewansiha, J. (2020). Evaluation of Antibacterial Potency of Citrus Limon (Lemon) Juice Against Some Pathogenic Organisms as Alternative Source of Chemotherapy. European Journal of Biology and Biotechnology, 1(1), 1-8.

Ezeabara, C.A., Okeke, C.U., Aziagba, B.O. (2013). Flavonoid Content of Citrus Species Grown in Awka, Anambra State, Shouthern Nigeria. International Journal of Agriculture and Biosciences 2(3), 103-107.

Ekawati, E.R., Darmanto, W. (2019). Lemon (Citrus limon) Juice Has Antibacterial Potential against Diarrhea-Causing Pathogen. IOP Conference Series: Earth and Environmental Science, 1-5.

Goswami, M., Pathak, V., Bharti, S.K., Chappalwar, A.M., Ojha, S., Singh,S., Singh, S. (2019). Effect of Low Temperature Preservation on Quality Characteristics of Meat. Journal of Animal Feed Science and Technology 7(1), 41-46.

Henderson, A.H., Fachrial, E., Lister, I.NE. (2018). Antimicrobial Activity of Lemon (Citrus limon) Peel Extract Against Escherichia coli. American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) 39 (1), 268-273.

Jufri, R.F. (2020). The Effect of Environmental Factors on Microbial Growth. Journal La Lifesci, 1(1), 12-17.

Knudsen, G. M., Sommer, H.M., Sorensen, N. D., Olsen, J. E., Aabo. (2011). S. Survival of Salmonella on cuts of beef carcasses subjected to dry aging. J Appl Microbiol 111(4), 848-854.

Mahdevi, S., Dehbokri, M. A., Hajazimian, S., Isazadeh, A. (2018). Contamination of Chicken Meat With Salmonella spp Distributed in Mahabad City, Iran. International Journal of Enteric Pathogens 6(3), 65-68.

Maturin, L, Peeler, J.T. (2001). Aerobic Plate Count. In: Bacteriological Analytical Manual Online. Center for Food Safety and Applied Nutrition. Washington DC (US): US Food and Drug Administration.

Moosavy, M.H., Hassanzadeh, P., Mohammadzadeh, E., Mahmoudi, R., Khatibi, S.A., Mardani, K. (2017). Antioxidant and Antimicrobial Activities of Essential Oil of Lemon (Citrus limon) Peel in Vitro and in a Food Model. Journal of Food Quality and Hazards Control, 4 : 42-48

Murali, N., Kumar-Philips, G.S., Rath, N.C., Marcy, J., Slavik, M.F. (2012). Effect of Marinating Chicken Meat with Lemon, Green Tea and Turmeric Against Foodborne Bacterial Pathogens. International Journal of Poultry Science. 11 (5) : 326-332.

Nidaullah, F., Omar, A.K.M., Rosma, A., Huda, N., Sohni, S. (2016). Analysis of Salmonella Contamination in Poultry Meat at Various Retailing, Different Storage Temperatures and Carcass Cuts - A Literature Survey. International Journal of Poultry Science 15 (3), 111-120.

Park, S.H., M. Aydin, A. Khatiwara, M.C. Dolan, D.F. Gilmore, J.L. Bouldin, S. Ahn and S.C. Ricke, (2014). Current and emerging technologies for rapid detection and characterization of Salmonella in poultry and poultry products. Food Microbiol 38, 250-262.

Prastiwi, S.S., and Ferdiansyah, F. (2013). Review Articles: Content and Pharmacology Activities of Lime (Citrus aurantifolia S.). Journal of Farmaka 15(2), 1-8.

Safitri, E., Hidayati, N.A., Hertati, R (2019). Prevalensi Bakteri Salmonella Pada Ayam Potong Yang Dijual Di Pasar Tradisional Pangkalpinang. Ekotonia: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi 4 (1), 25-30.

Shafini, A.B., Son, R., Mahyudin, N.A., Rukayadi, Y. and Zainazor TC. (2017). Prevalence of Salmonella sp. in Chicken and Beef from Retail Outlets in Malaysia. International Food Research Journal 24(1), 437-449

Sartika, D., Susilawati, Arfani, G. (2016). Identification of Salmonella Sp. Contamination on Broilers with Quantification Method at Three Traditional Markets and Two Modern Markets in Bandar Lampung. Jurnal Teknologi Industri & Hasil Pertanian 21 (2), 89-96.

Thames, H,T., Sukumaran, H,T. (2020). A Review of Salmonella and Campylobacter in Broiler Meat: Emerging Challenges and Food Safety Measures. Foods 2020, 9 (6), 1-22.

Zelpina, E., Purnawarman, T., Lukman, D.W. (2018). Keberadaan Salmonella Sp. Pada Daging Ayam Suwir Bubur Ayam Yang Dijual Di Lingkar Kampus Institut Pertanian Bogor Dramaga Bogor. Jurnal Penelitian Pascapanen Pertanian 15 (2), 73-79.




DOI: https://doi.org/10.32528/bioma.v6i2.5879

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