Aktivitas Enzim Hidrolase pada Penapisan Isolat Actinomycetes Kandidat Probiotik Udang

Sumardi Sumardi, Vanya Qatrunada, Salman Farisi, Achmad Arifiyanto, Christina Nugroho Ekowati

Abstract


Actinomycetesis one of the microorganisms which have the ability of enzymes hydrolases such as cellulose, amylase, protease, and manannase. Manufacturer of enzymes of the hydrolase can be used as a candidate probiotic. A candidate probiotic should have a good resistance in different environmental conditions including pH and salinity.  This research is conducted to filter the candidate’s probiotics from bacteria Actinomycetes that tolerance salinity and has the activity of the enzyme hydrolase. Testing the activity of the enzyme was preformed qualitatively on the variation of pH 4, 7, and 9.8.  Theability of Atinomycetesproduce enzymes hydrolases is indicated by the colony on media containing the substrate.From the test results, cellulase enzyme activity with the largest enzymatic index value is 3.5 AF2 isolates pH 9.8. Amylase enzyme activity with the largest enzymatic index value is 3.66 RH1 isolates pH 7. Protease enzyme activity with the largest enzyme index value is 0.66, AF2 isolates at pH 7. Mananase enzyme activity with the largest enzymatic index value is 0.85, namely AF2 isolates pH 4. In testing the effect of salinity test isolates were able to grow at variations in the concentration of NaCl 0, 3, and 6%.

Keywords


Actinomycetes, Cellulotytic, Amylolytic, Proteolytic, Mananolytic, Salinity

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References


Arifiyanto, A., Surtiningsih, T., Ni’matuzahroh, Fatimah, Agustina, D., & Alami, N. H. (2020). Antimicrobial activity of biosurfactants produced by Actinomycetes isolated from rhizosphere of Sidoarjo mud region. Biocatalysis and Agricultural Biotechnology, 24. https://doi.org/10.1016/j.bcab.2020.101513

Arji, M., Rezky W., Nurhalisa W. O., dan Yanti N. A. 2018. Isolatsi , Karakterisasi, Dan Uji Potensi Bakteri Penghasil Enzim Termostabil Dari Sumber Air Panas Lainea, Sulawesi Tenggara. Biowallace,5 (1)

Asnani A., Ryandini D., dan Suwardi.2016.Screening of marine Actinomycetes from segara Anakan for natural pigment and hydrolytic activites. IOP Conference Serie: Mateirals science and Engineering

Apun, K., Jong, B.C., and Salleh, M.A., 2000, Screening and Isolation of a Cellulolytic and Amylolitic Bacillus from Sago Pith Waste, J. General Applied Microbiology, 46:263-267

Chau N.T.T, Hieu. N.X., Thuan L.T.N , Matsumoto M. And Miyajima M. 2011. Identification and Characterization of Actinomycetes Antagonistic to Pathogenic Vibrio spp. Isolated from Shrimp Culture Pond Sediments in Thua Thien Hue–Viet Nam. J. Fac. Agr. Kyushu Univ.

Das, S., Lyla, P. S., and Khan, A. S. 2006. Application of Streptomyces as a probiotic in the laboratory culture of Penaeus monodon (Fabricius). Israeli Journal of Aquaculture, 58(3), 198–204.

Das, S., Ward, L. R., and Burke, C.2010. Screenng of streptomyces spp. for potential us as Probiotics in aquaculture. Aquaculture 305

De Souza. I. A., Orsi D. C., Gomes A. J., and Lunardi C. N. 2019. Enzymatic hydrolysis of starch into sugar is influenced by microgel assembly. Biotechnology Reports.

Djohan A. C., 2012. Isolatsi Dan Identifikasi Bakteri Manilitik Laut Dari Pulau Pari. IPB. Bogor (Tesis).

Faturrahman and Widiyanti, 2012. Seleksi Parsial Vibrio spp. Kandidat Probiotik: Vialibitas Pada Berbagai Kondisi Suhu, pH, Dan Salinitas. Jurnal biologi XVI (2),36-40, Universitas Mataram

Gebreyohannes G. 2015. Isolation and optimization of amylase producing bacteria and actinomycetes from soil samples of Maraki and Tewedros campus, University of Gondar, North West Ethiopia African Journal of Microbiology Research

Irwan, A.w. dan F.Y. Wicaksono. 2017. Perbandingan Pengukuran Luas daun Kedelai dengan Metode Gravimetri, Regresi dan Scenner. Jurnal Kultivasi. 16.

Jaradat, Z.,Dawagreh,A.,Ababneh,Q., and Saadoun,I.2008.Influence of culture conditionson cellulose production by Streptomyces sp. (StrainJ2). Jordan J.Biol.Sci.1,141–146.

Karthikeyan K,. Bhavini. S. G. and Jayanthi G. 2015. Isolation and screening of marine bacteria and Actinomycetes for enzyme production and antibacterial activity from Vadinar coast,Jamnagar, Gulf of Kachchh. Current Research in Microbiology and Biotechnology 3 (6), 800-804

Kulkarni C.P. and Maurya C.B. 2017. Characterization of the Cellulase Enzyme Produced by Actinomycetes Isolated from the Mangrove Coastal. Biosciences Biotechnology Research Asia.14 (2):685-690

Nurkanto A.2017. Studi Kelimpahan Aktinomisetes Tanah dan Hubungannya TerhadapEnzim Selulase, Amilase, Total Karbon dan Nitrogen Hutan PascaKebakaran Bukit Bangkirai Kalimantan Timur. Bidang Mikrobiologi. Jurnal Biologi Indonesia.5

Rakhfid A., Halida W. O., Rochmady, dan Fendy. 2018. Aplikasi Probiotik untuk Pertumbuhan Hidup udang vaname iLitopenaeus vannamei Pada Padat Tebar Berbeda. Jurnal Akuatikisle. 2 (2), 41-48

Sastrahidayatdan I. Rochdjatun. 2014. Peranan Mikroba bagi KesehatanTanaman dan Kelestarian Lingkungan. Universitas Brawijaya Press

S. Evan, A. Meryandini and T. C. Sunarti. 2020. Characteristics of mannanase enzyme in

actinomycetes isolated from Taman Nasional Bukit Duabelas Jambi. IOP Conference Series: Earth and Environmental Science. 457

Sunaryanto R, Martius E, and Marwoto B. 2014. Uji Kemampuan Lactobacillus casei sebagai agensia probiotik Ability Test of Lactobacillus Casei as A Probiotic Agents. J Bioteknoligi dan Biosains Indonesia.

Sumardi. 2007. Isolasi Dan Karakterisasi Mananase Ekstrasaluler dari Fusarium oxysporum. Jurnal sains Mipa. 12 (1), 43-48.

Tan LT-H., Chan K-G., Lee L-H., dan Goh B-H. 2016. Streptomyces bacteria as potential probiotics in aquaculture. Front. Microbiol

Vonothini G., Murugan M., Sivakumar K and Sudha S. 2008. Optimization Of Protease Production By An Actinomycete Strain, PS-18A Isolated From An Estuarine Shrimp Pond . J. Biotechnol.

Yusriah dan N. D. Kuswytasari. 2016. Pengaruh pH dan Suhu Terhadap Aktivitas Protease

Penicillium sp. Jurnal Sain dan Seni Pomits 2




DOI: https://doi.org/10.32528/bioma.v6i1.3548

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